Barker J M
Transfusion. 1982 Nov-Dec;22(6):507-10. doi: 10.1046/j.1537-2995.1982.22683068614.x.
The serologic reactivity of a monoclonal anti-C3d and commercial "monospecific" anti-C3 and polyspecific antiglobulin reagents was compared. The testing consisted of a direct antiglobulin test performed on donor and patient cells and on in vivo and in vitro complement-coated cells; an indirect antiglobulin test was performed with low-ionic-strength solution crossmatches. The monoclonal anti-C3d demonstrated reactivity very similar to that of the commercial heterogeneous rabbit anti-C3d; it gave relatively few weak "false positive" reactions with normal donor and patient red blood cells, and comparably strong reactions with in vitro and in vivo C3d-coated red blood cells. The results suggest a promising future for monoclonal antiglobulin reagents.
比较了一种单克隆抗C3d与市售“单特异性”抗C3及多特异性抗球蛋白试剂的血清学反应性。检测包括对供体和患者细胞以及体内和体外补体包被细胞进行的直接抗球蛋白试验；采用低离子强度溶液交叉配血法进行间接抗球蛋白试验。单克隆抗C3d表现出与市售异质性兔抗C3d非常相似的反应性；它与正常供体和患者红细胞产生相对较少的弱阳性“假阳性”反应，而与体外和体内C3d包被的红细胞产生相当强的反应。结果表明单克隆抗球蛋白试剂具有广阔的前景。
