Zan-Bar I, Barzilay M
Eur J Immunol. 1982 Oct;12(10):838-44. doi: 10.1002/eji.1830121008.
The role of IgD (delta) in the induction of tolerance in murine B lymphocytes was explored in the in vivo adoptive antibody response to bovine serum albumin (BSA). Delta+ or delta- B cells purified on the fluorescence-activated cell sorter were injected, with or without purified T cells, into lethally irradiated intermediate hosts, which were rendered tolerant by an injection of deaggregated BSA one day after the cell transfer. Spleen cells from the intermediate hosts were treated with anti-Thy-1.2 antiserum and complement mixed with normal T cells and were transferred to final hosts. The ability of the B cells of the final hosts to respond to the tolerogen BSA and to dinitrophenylated human gamma globulin antigen was examined. This approach enabled examination of the function of the B cell subpopulations in an environment free of tolerogen and of induced T suppressor cells. The results revealed that in the presence of T cells the delta+ subpopulation was highly resistant to tolerance induction, whereas the delta- subpopulation was highly susceptible to tolerance induction. However, there was no difference in susceptibility to tolerance induction between the two subpopulations when tolerance was induced in the absence of T cells.
在对牛血清白蛋白（BSA）的体内过继性抗体应答中，研究了IgD（δ）在诱导小鼠B淋巴细胞耐受性方面的作用。通过荧光激活细胞分选仪纯化的δ⁺或δ⁻ B细胞，在有或无纯化T细胞的情况下，注入经致死剂量照射的中间宿主，这些中间宿主在细胞转移后一天通过注射去聚合的BSA而产生耐受性。来自中间宿主的脾细胞用抗Thy-1.2抗血清和补体处理，并与正常T细胞混合，然后转移到最终宿主。检测最终宿主的B细胞对耐受原BSA和二硝基苯基化人γ球蛋白抗原作出反应的能力。这种方法能够在无耐受原和诱导性T抑制细胞的环境中检测B细胞亚群的功能。结果显示，在有T细胞存在的情况下，δ⁺亚群对耐受性诱导具有高度抗性，而δ⁻亚群对耐受性诱导高度敏感。然而，在无T细胞诱导耐受性时，两个亚群在耐受性诱导敏感性方面没有差异。
