Recognition of self and non-self tissue antigens in peripheral blood leucocytes from normal rats detected by the tube LAI assay.

Adherence to glass of peripheral blood leucocytes from normal, non-immunized rats was measured in the tube LAI assay in the presence of extracts isolated from tissues of syngeneic or allogeneic donors. Adherence inhibition was significantly higher when peripheral blood leucocytes were incubated with syngeneic than with allogeneic tissue extracts. The results indicate that peripheral blood leucocytes from normal, non-immunized animals are capable of recognizing self and non-self tissue antigens and that this recognition can be detected by the tube LAI assay.