Kalafut F, Novotná L, Koníková E
Neoplasma. 1980;27(4):373-7.
Leukocytes of peripheral blood of F1 hybrid inbred strain of rats LW X AVN and rats of inbred Lewis strain, immunized for three consecutive weeks with increasing doses of live or dead MC-1 or B 77 tumor cells, incubated for 20 hours with specific tumor extract, showed a lower adhering ability (LAI 32.8 +/- 16.6, 44.4 +/- 14.0, 43.1 +/- 7.4%) than that of the same cell population cultured without a specific antigen. The nonspecific tumor extract did not produce any LAI reactivity (4.4 +/- 5.9, 5.8 +/- 8.2, 6.7 +/- 5.9%). The values of LAI leukocytes of the controls tested by both the antigens were concordant with those found in samples of the same cell population tested without any antigens. The discussion bears on a possibility of applying the 20-hour modification of the LAI test in studies of cell immunity in immunized patients.
将大鼠LW X AVN的F1杂交近交系和近交Lewis系大鼠的外周血白细胞，用递增剂量的活的或死的MC - 1或B 77肿瘤细胞连续免疫三周，再与特异性肿瘤提取物孵育20小时，结果显示其黏附能力（白细胞黏附抑制率LAI分别为32.8 +/- 16.6、44.4 +/- 14.0、43.1 +/- 7.4%）低于未用特异性抗原培养的相同细胞群体。非特异性肿瘤提取物未产生任何LAI反应性（4.4 +/- 5.9、5.8 +/- 8.2、6.7 +/- 5.9%）。两种抗原检测的对照组LAI白细胞值与未用任何抗原检测的相同细胞群体样本中的值一致。讨论涉及在免疫患者的细胞免疫研究中应用LAI试验20小时改良法的可能性。
