用偶氮胂III测定的人红细胞膜空壳钾通透性相关的细胞内钙离子变化。
Changes of intracellular Ca++ as measured by arsenazo III in relation to the K permeability of human erythrocyte ghosts.
作者信息
Yingst D R, Hoffman J F
出版信息
Biophys J. 1978 Sep;23(3):463-71. doi: 10.1016/S0006-3495(78)85462-9.
Abstract
A Ca-sensitive dye, arsenazo III, has been incorporated into resealed human erythrocyte ghosts and calibrated to monitor continuously micromolar concentrations of intracellular ionized Ca ([Ca++]i). When the external concentration of Ca is much greater than [Ca++]i, [Ca++]i increases because of a net balance between Ca influx and efflux. Dynamic changes in [Ca++]i regulate K efflux, which in turn may influence the rate of Ca influx. A procedure for purifying arsenazo III is also described.
摘要
一种钙敏感染料偶氮胂III已被掺入重新封闭的人红细胞影泡中，并经过校准以连续监测细胞内游离钙离子（[Ca++]i）的微摩尔浓度。当外部钙离子浓度远高于[Ca++]i时，由于钙流入和流出之间的净平衡，[Ca++]i会增加。[Ca++]i的动态变化调节钾离子流出，而钾离子流出反过来可能影响钙流入的速率。本文还描述了一种纯化偶氮胂III的方法。
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