Stern M H, Dreizen S, Mackler B F, Levy B M
J Dent Res. 1982 Dec;61(12):1408-12. doi: 10.1177/00220345820610120601.
Twelve histologically-confirmed periapical granulomas were evaluated by conventional immunologic rosette assays for the presence of T-lymphocytes and complement receptor-bearing lymphocytes. A technique for dispersing the granuloma cells into suspensions was adopted to facilitate performance of the assays which were not applicable to tissue sections. Differential cell counts by an acridine orange vital dye method disclosed that the cell suspensions contained 30% macrophages, 44% lymphocytes, 15% plasma cells, and 12% neutrophils. Complement receptor-bearing cells comprised 17.9%, and T cells comprised 34.5% of the unseparated inflammatory cells. This study provides the first direct evidence of a predominance of thymic-derived lymphocytes in the lymphocyte compartment of the periapical granuloma. Analysis of the data shows that cell-mediated immunity most likely plays a role in the pathogenesis of the periapical granuloma.
通过传统免疫玫瑰花结试验对12例经组织学确诊的根尖周肉芽肿进行评估，以检测T淋巴细胞和携带补体受体的淋巴细胞的存在情况。采用一种将肉芽肿细胞分散成悬液的技术，以利于进行不适用于组织切片的试验。通过吖啶橙活体染色法进行的细胞分类计数显示，细胞悬液中含有30%的巨噬细胞、44%的淋巴细胞、15%的浆细胞和12%的中性粒细胞。携带补体受体的细胞占未分离炎性细胞的17.9%，T细胞占34.5%。本研究首次直接证明了根尖周肉芽肿淋巴细胞区室中胸腺来源淋巴细胞占优势。数据分析表明，细胞介导的免疫很可能在根尖周肉芽肿的发病机制中起作用。
