Characterization of androgen uptake by purified nuclei from an androgen-dependent and two androgen-independent cell lines of the Shionogi mouse mammary carcinoma.

Properties of androgen uptake by nuclei prepared from an androgen-dependent tumour cell line occurred after 2 h incubation at 20 degrees C; 2. Nuclei from three tumour cell lines displayed similar affinity for DHT but the two androgen-independent cell lines had less than one-quarter the number of uptake sites; 3. Loss of label from nuclei which had been pre-incubated with [3H]-DHT for 18 h at 20 degrees C was greater from the AD cell line nuclei than from the androgen-independent cell lines; 4. Whole cell contamination of the nuclear preparations did not contribute to specific DHT uptake.