Chang M P, Strehler B L, Makinodan T
Mech Ageing Dev. 1982 Sep;20(1):65-73. doi: 10.1016/0047-6374(82)90075-6.
The effects of 2-mercaptoethanol and age on spleen cells derived from young (3-4 months) and old (24-months) C57BL/6 mice were measured with respect to T cell growth factor or Interleukin 2 production. It was shown that: (A) 2-mercaptoethanol (or some homologue) is absolutely required for T cell growth factor production in vitro by murine cells (the optimum concentration is 5 X 10(-5) M for both young and old cells); (B) old cells are less responsive to suboptimum concentrations than young cells but their responses are not reduced to the same degree as young cells by supraoptimum toxic doses of 2-mercaptoethanol; and (C) at optimum 2-mercaptoethanol concentrations young and old cells have similar kinetic responses for T cell growth factor production and the accumulation of the T cell growth factor reaches a maximum between 18 and 24 hours. Considerations are presented of 2-mercaptoethanol (or some homologue) in its role as an important reactant in the production of T cell growth factor and in its susceptibility to aging.
就T细胞生长因子或白细胞介素2的产生而言，检测了2-巯基乙醇和年龄对来自年轻（3 - 4个月）和年老（24个月）C57BL/6小鼠的脾细胞的影响。结果表明：（A）2-巯基乙醇（或某些同系物）是鼠细胞体外产生T细胞生长因子所绝对必需的（年轻和年老细胞的最佳浓度均为5×10⁻⁵ M）；（B）年老细胞对次最佳浓度的反应比年轻细胞弱，但超最佳毒性剂量的2-巯基乙醇对它们反应的降低程度不如对年轻细胞的降低程度；（C）在最佳2-巯基乙醇浓度下，年轻和年老细胞产生T细胞生长因子的动力学反应相似，并且T细胞生长因子的积累在18至24小时之间达到最大值。文中讨论了作为T细胞生长因子产生中的重要反应物及其对衰老易感性的2-巯基乙醇（或某些同系物）。
