Zernov I N, Stefani D V, Vel'tishchev Iu E
Biull Eksp Biol Med. 1978 Sep;86(9):339-41.
Rabbit antilymphocytic serum and complement were taken in quantities provoking the death of 50 per cent of human lymphocytes secreted in the phycol-verographin density gradient. Compounds (dinatrium salt of oxyethylendiphosphonate acid, alkylated oxyethylendiphosphonate acid, aminoethyloxyethylendiphosphonate acid, aminobenzyldiphosphonate, and aminoisopropyldiphosphonate acids) of 10 mM diphosphonic composition solutions in quantities of from 0.001 ml to 0.2 ml were added to experimental samples of 0.2 ml lymphocytes suspension containing 2.4 X 10(4)--6.0 X 10(4) cells. A decrease in the dead cell count in staining with a 0.1 per cent trypan blue solution was observed. Dinatrium salt of oxyethylendiphosphonate acid and alkylated oxyethylendiphosphonate acid proved to be most effective: it showed protective properties already in a dose of 0.01 ml. All the tested components had a pronounced protective action in a dose of 0.1 ml.
取能致使在藻红蛋白密度梯度中分泌的50%人淋巴细胞死亡量的兔抗淋巴细胞血清和补体。将10 mM二膦酸组合物溶液（氧乙烯二膦酸二钠、烷基化氧乙烯二膦酸、氨基乙氧基乙烯二膦酸、氨基苄基二膦酸和氨基异丙基二膦酸），以0.001 ml至0.2 ml的量添加到含有2.4×10⁴ - 6.0×10⁴个细胞的0.2 ml淋巴细胞悬液的实验样本中。观察到用0.1%台盼蓝溶液染色时死细胞计数减少。氧乙烯二膦酸二钠和烷基化氧乙烯二膦酸被证明是最有效的：在0.01 ml剂量时就已显示出保护特性。所有测试成分在0.1 ml剂量时都有明显的保护作用。
