Zernov I N, Stefani D V, Vel'tishchev Iu E
Biull Eksp Biol Med. 1979 Mar;87(3):259-60.
Rabbit antilymphocyte serum (ALS) and complement were used in doses killing 50% of lymphocytes (staining with a 1% solution of trypan blue). In rosette-forming and blast-cell transformation reactions the counts of rosette forming and transformed cells were 60 and 24% respectively, as compared to those in controls. After adding 0.1 ml of 10 mM solution of potassium hydroxyethylene-diphosphonate belonging to a group of synthetic diphosphonates, the protective effect was observed shown by that the counts of rosette-forming and transformed lymphocytes did not differ from the control ones, wherein the reactions were produced with intact cells.
兔抗淋巴细胞血清（ALS）和补体的使用剂量能杀死50%的淋巴细胞（用1%台盼蓝溶液染色）。在玫瑰花结形成反应和母细胞转化反应中，与对照组相比，玫瑰花结形成细胞和转化细胞的计数分别为60%和24%。加入0.1 ml属于合成二膦酸盐组的10 mM羟基亚乙基二膦酸钾溶液后，观察到了保护作用，表现为玫瑰花结形成淋巴细胞和转化淋巴细胞的计数与对照组无异，其中反应是用完整细胞进行的。
