通过插入合适的膜成分，无反应性淋巴细胞获得促有丝分裂反应性。
Acquisition of mitogenic responsiveness by nonresponding lymphocytes upon insertion of appropriate membrane components.
作者信息
Jakobovits A, Sharon N, Zan-Bar I
出版信息
J Exp Med. 1982 Oct 1;156(4):1274-9. doi: 10.1084/jem.156.4.1274.
Abstract
The effect of insertion of plasma membrane components from lymphocytes responding to mitogens into the membranes of nonresponding cells using Sendai virus envelopes as vehicles was examined. T cells modified by B membranes were stimulated by lipopolysaccharide (LPS) to proliferate as well as to produce interleukin-2 activity. B cells modified by T membranes were stimulated by concanavalin A to proliferate and to produce interleukin-2 activity. B cells derived from C3H/HeJ LPS-nonresponder strain of mice, when modified by B membranes derived from the LPS-responder C3H/eb strain, acquired LPS responsiveness. These findings indicate that the inability of either T or B cells to respond to specific mitogens is due to the lack of suitable plasma membrane constituents and that by changing the membrane composition the lymphocytes can be endowed with new functions.
摘要
研究了以仙台病毒包膜为载体，将对丝裂原产生反应的淋巴细胞的质膜成分插入无反应细胞的膜中所产生的效应。经B细胞膜修饰的T细胞受到脂多糖（LPS）刺激后增殖，并产生白细胞介素-2活性。经T细胞膜修饰的B细胞受到刀豆球蛋白A刺激后增殖，并产生白细胞介素-2活性。源自C3H/HeJ LPS无反应小鼠品系的B细胞，当用源自LPS反应性C3H/eb品系的B细胞膜进行修饰时，获得了LPS反应性。这些发现表明，T细胞或B细胞对特定丝裂原无反应的原因是缺乏合适的质膜成分，并且通过改变膜成分，淋巴细胞可以获得新的功能。
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2
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3
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Proc Natl Acad Sci U S A. 1980 Dec;77(12):7247-51. doi: 10.1073/pnas.77.12.7247.
4
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Nature. 1966 Jan 29;209(5022):521-3. doi: 10.1038/209521b0.
5
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Exp Cell Res. 1975 Dec;96(2):374-82. doi: 10.1016/0014-4827(75)90270-0.
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