经神经氨酸酶处理的人淋巴细胞膜蛋白。
Human lymphocyte membrane proteins treated with neuraminidase.
作者信息
Gmünder H, Lerch P, Lesslauer W
出版信息
Biochim Biophys Acta. 1982 Dec 22;693(2):359-63. doi: 10.1016/0005-2736(82)90443-6.
Human peripheral blood lymphocytes were surface-iodinated, treated with neuraminidase from Vibrio cholerae and lysed with non-ionic detergent. In addition, surface membrane fractions were isolated from surface-iodinated cells in the absence of detergents and treated with neuraminidase after membrane isolation. The effect of neuraminidase treatment on the membrane proteins was studied by two-dimensional gel electrophoresis. One surface-labelled protein of 45 000 molecular weight which is characterized by its association with the detergent-resistant matrix of the cells and by its specific enrichment in an isolated membrane fraction, was found to be particularly sensitive to neuraminidase treatment both of intact cells and isolated membranes. A prominent labelled protein of apparent molecular weight of 60 000 is observed in the soluble fraction after neuraminidase treatment of intact cells. The analogous protein is detected when isolated membrane fractions are treated with neuraminidase.
人外周血淋巴细胞经表面碘化处理，用霍乱弧菌神经氨酸酶处理，并用非离子去污剂裂解。此外，在无去污剂的情况下从表面碘化细胞中分离出表面膜组分，并在膜分离后用神经氨酸酶处理。通过二维凝胶电泳研究神经氨酸酶处理对膜蛋白的影响。发现一种分子量为45000的表面标记蛋白，其特征在于与细胞的抗去污剂基质相关联，并在分离的膜组分中特异性富集，该蛋白对完整细胞和分离膜的神经氨酸酶处理都特别敏感。完整细胞经神经氨酸酶处理后，在可溶组分中观察到一种明显分子量为60000的显著标记蛋白。当分离的膜组分用神经氨酸酶处理时，可检测到类似的蛋白。
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