低强度脉冲超声对两栖类胚胎组织的作用。

The action of low-intensity pulsed ultrasound on amphibian embryonic tissues.

作者信息

Sarvazyan A P, Beloussov L V, Petropavlovskaya M N, Ostroumova T V

出版信息

Ultrasound Med Biol. 1982;8(6):639-54. doi: 10.1016/0301-5629(82)90121-1.

Abstract

Amphibian embryos at different stages of development, and ectomesodermal explants (small pieces of embryonic tissue extirpated from lateral parts of the embryos just after neurulation), have been irradiated with continuous and pulsed ultrasound at a frequency of 0.88 MHz, spatial and temporal average intensities being within the range 0.025-0.1 W cm2. Ultrasound pulse repetition frequencies were varied within 10-1000 Hz, duty factor being constant and equal to 0.5. Destruction of embryonic tissue as an immediate effect, and interruption or distortion of development as delayed (24 hr) effects, were registered. Significant damaging effects of non-cavitational and non-thermal origin and dependent on pulse repetition frequencies, were observed. Highly frequency-dependent resonance-type effects were obtained for irradiated explants. The maximum effect on Rana temporaria explants was obtained at pulse repetition frequencies of 10-20 Hz and on Xenopus laevis explants at 110-130 Hz.

摘要

对处于不同发育阶段的两栖类胚胎以及外胚层中胚层外植体（在神经胚形成后立即从胚胎侧面切除的小块胚胎组织），用频率为0.88MHz的连续和脉冲超声进行辐照，空间和时间平均强度在0.025 - 0.1W/cm²范围内。超声脉冲重复频率在10 - 1000Hz范围内变化，占空因数恒定且等于0.5。记录了作为即时效应的胚胎组织破坏以及作为延迟（24小时）效应的发育中断或畸变。观察到了非空化和非热起源且依赖于脉冲重复频率的显著损伤效应。对于辐照的外植体，获得了高度依赖频率的共振型效应。在脉冲重复频率为10 - 20Hz时对林蛙外植体产生最大效应，在110 - 130Hz时对非洲爪蟾外植体产生最大效应。