Mikhailov A T, Gorgolyuk N A, Tacke L, Mykhoyan M M, Grunz H
Laboratory of Organogenesis, Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow.
Int J Dev Biol. 1995 Apr;39(2):317-25.
A high neuralizing activity has been determined in forebrain of 7.5-day old chick embryos using Rana temporaria early gastrula ectoderm as reacting tissue (Mikhailov and Gorgolyuk, Soviet Scientific Reviews, Section of Physiology and General Biology, Vol. 1: 267-306, 1987). The corresponding protease-sensitive agent was extracted, partially purified by chromatography on DEAE-Toyopearl and Heparin-Ultragel columns, and its neuralizing activity was tested in vitro on ectoderm isolated from early gastrulae of R. temporaria, Triturus alpestris, and Xenopus laevis at different concentrations and for different periods of time (animal cap assay). Induction of neural structures was found in R. temporaria and T. alpestris explants (up to 100 and 60%, respectively), but not in cultures of X. laevis ectoderm. Under our experimental conditions, so-called "autoneuralization" of the ectoderm explants can safely be excluded. The results are discussed in relation to the neural competence of amphibian ectoderm and the mechanisms of neuralizing actions of different factors which might be involved in neural induction and patterning.
利用欧洲林蛙早期原肠胚外胚层作为反应组织，已测定出7.5日龄鸡胚前脑具有较高的神经诱导活性（米哈伊洛夫和戈尔戈柳克，《苏联科学评论》，生理学与普通生物学部分，第1卷：267 - 306页，1987年）。提取了相应的蛋白酶敏感剂，通过在DEAE - 托普雷尔和肝素 - 超凝胶柱上进行层析进行部分纯化，并在体外以不同浓度和不同时间段对从欧洲林蛙、高山蝾螈和非洲爪蟾早期原肠胚分离的外胚层进行其神经诱导活性测试（动物帽试验）。在欧洲林蛙和高山蝾螈外植体中发现了神经结构的诱导（分别高达100%和60%），但在非洲爪蟾外胚层培养物中未发现。在我们的实验条件下，可以安全地排除外胚层外植体的所谓“自动神经化”。结合两栖动物外胚层的神经感受态以及可能参与神经诱导和模式形成的不同因子的神经诱导作用机制对结果进行了讨论。
