Coppo R, De Marchi M, Basolo B, Martina G, Rollino C, Boero R, Giraudo G, Rossano C, Quarello F, Piccoli G
Arch Sci Med (Torino). 1982 Oct-Dec;139(4):381-92.
In the last years a few tests for the detection of circulating immune complexes (CIC) have been published. Nevertheless the data concerning their sensitivity and reproductibility are often partial. The Authors have here analysed, following this point of view, two methods for the detection of CIC, based on different mechanisms and therefore able to identify different kinds of CIC; the tests are: the PEG precipitation test and the C1q solid phase method. The reproductibility of the two methods is good, even when the samples have been many times thawn or kept at 20 degrees C for a few weeks; C1q test sensibility appears distinctly better than PEG test sensibility. By the two methods, but particularly with the PEG, it is possible to identify fluctuation of the IC levels during the day. The contemporaneous use of these two techniques can give results really useful to clinically monitorize some immune complexes diseases, such as many human glomerulonephrites.
在过去几年里，已经发表了一些用于检测循环免疫复合物（CIC）的试验。然而，关于其敏感性和可重复性的数据往往不完整。从这一角度出发，作者在此分析了两种基于不同机制检测CIC的方法，因此能够识别不同类型的CIC；这两种试验分别是：聚乙二醇（PEG）沉淀试验和C1q固相法。即使样本多次解冻或在20摄氏度下保存数周，这两种方法的可重复性也很好；C1q试验的敏感性明显优于PEG试验的敏感性。通过这两种方法，尤其是使用PEG，可以识别一天中IC水平的波动。同时使用这两种技术能够得出对临床监测某些免疫复合物疾病（如许多人类肾小球肾炎）非常有用的结果。
