Van Dijk H, Van Voorst J, Jankowski I G, Imhof J W, Kerckhaert J A
Clin Exp Immunol. 1984 Aug;57(2):495-501.
Sera were, under strictly standardized conditions, centrifuged in the presence of 0-5% PEG and the Clq concentration in the precipitates was measured by radial immunodiffusion. The presence of circulating immune complexes and rheumatoid factor(s) resulted in a shift of Clq precipitation to lower PEG concentrations. Clq precipitation at 1.5% PEG was shown to be specific for sera containing immune complexes. Under similar conditions addition of aggregated IgG to normal human serum gave rise to Clq precipitation directly proportional to the amount of aggregated IgG. Precipitation of endogenous Clq at 1.5% PEG and assay by radial immunodiffusion may therefore be useful for the detection and quantitation of immune complexes in human serum.
在严格标准化的条件下，将血清在含有0 - 5%聚乙二醇（PEG）的情况下进行离心，沉淀物中的Clq浓度通过放射免疫扩散法测定。循环免疫复合物和类风湿因子的存在导致Clq沉淀向较低PEG浓度偏移。结果表明，1.5% PEG时的Clq沉淀对含有免疫复合物的血清具有特异性。在类似条件下，向正常人血清中加入聚集的IgG会引起与聚集IgG量成正比的Clq沉淀。因此，1.5% PEG时内源性Clq的沉淀及放射免疫扩散测定法可能有助于检测和定量人血清中的免疫复合物。
