Wollman Y, Barak Y, Lewin S
Biomed Pharmacother. 1982;36(6-7):323-5.
Bacterial endotoxin was detected by the Limulus Amebocyte Lysate (LAL) assay in four preparations of human placental granulocyte-macrophage colony-stimulating-factor (HP-CSF), a humoral factor required for the production of human granulocyte-macrophage colonies in semi-solid cultures. The complete removal of endotoxin from these preparations was carried out by its adsorption to LAL proteins, either directly of following coupling to sepharose beads. Removal of endotoxin from HP-CSF did not result in any reduction of its in vitro granulopoietic activity, and the LAL-sepharose complex could be recycled for further use.
采用鲎试剂法对四种人胎盘粒细胞巨噬细胞集落刺激因子（HP-CSF）制剂进行细菌内毒素检测，HP-CSF是一种在半固体培养中产生人粒细胞巨噬细胞集落所需的体液因子。通过将内毒素吸附到鲎试剂蛋白上，直接或在与琼脂糖珠偶联后，可将这些制剂中的内毒素完全去除。从HP-CSF中去除内毒素不会导致其体外粒细胞生成活性的任何降低，并且鲎试剂-琼脂糖复合物可循环再利用。
