Sansoni P, Rossetti A, Tridente G, Butturini U
J Immunopharmacol. 1982;4(3):223-32. doi: 10.3109/08923978209026436.
The effect of various concentrations (0.015 - 10 micrograms/ml) of Levamisole (LMS) on the peripheral lymphocytes of patients with malignant lymphomas (ML) and normal donors was investigated in vitro. The parameters studied include: E rosettes forming cells (total T lymphocytes), active E rosettes (early T lymphocytes) and DNA synthesis induced by pitogens PHA and Con A. LMS improved significantly lymphocyte response both in patients with ML and normal donors when the cells were stimulated by Con A. In both groups no significant effect was observed on the response to PHA nor on the percentage of E-rosettes, whereas the mean number of active E rosettes was significantly increased on all concentrations of the drug. While in the normal subjects a positive statistical correlation between active E-rosettes and Con A response was observed, in patients with ML an inverse correlation was found. This latter correlation was partially reversed by LMS. These results indicate that LMS, both in normals and in patients with ML, interacts with a specific cell subset (early rosetting lymphocytes), with different functions in normal and pathological situations. The drug shows an effect on the immunological handicap of lymphocytes of patients with ML.
体外研究了不同浓度（0.015 - 10微克/毫升）的左旋咪唑（LMS）对恶性淋巴瘤（ML）患者和正常供体外周淋巴细胞的影响。研究的参数包括：E花环形成细胞（总T淋巴细胞）、活性E花环（早期T淋巴细胞）以及促有丝分裂原PHA和Con A诱导的DNA合成。当细胞受到Con A刺激时，LMS显著改善了ML患者和正常供体的淋巴细胞反应。在两组中，未观察到对PHA反应或E花环百分比有显著影响，而在所有药物浓度下，活性E花环的平均数量均显著增加。在正常受试者中，活性E花环与Con A反应之间存在正相关统计关系，而在ML患者中则发现呈负相关。LMS部分逆转了后一种相关性。这些结果表明，LMS在正常人和ML患者中均与特定细胞亚群（早期花环形成淋巴细胞）相互作用，在正常和病理情况下具有不同功能。该药物对ML患者淋巴细胞的免疫缺陷有影响。
