Ramsay G, Hayman M J, Bister K
EMBO J. 1982;1(9):1111-6. doi: 10.1002/j.1460-2075.1982.tb01305.x.
The putative transforming proteins of the four acute leukaemia viruses belonging to the MC29 subgroup were shown to be phosphorylated in vivo. Comparison of the MC29 and CM11 encoded phosphoproteins revealed identical tryptic phosphopeptide maps, with both the gag and myc domains being phosphorylated. In contrast, the MH2 phosphoprotein was only phosphorylated on the gag domain. Analysis of partial transformation-defective MC29 deletion mutants revealed that the deletions had removed the v-myc specific phosphopeptides. Phosphoamino acid analysis showed that these deleted phosphopeptides were phosphorylated on threonine. Moreover, a back mutant that had regained transforming ability had regained these phosphopeptides. These studies correlate the phosphorylation of the gag-myc protein with the transformation capability of the virus.
属于MC29亚组的四种急性白血病病毒的假定转化蛋白在体内被证明是磷酸化的。对MC29和CM11编码的磷蛋白的比较显示，胰蛋白酶磷酸肽图谱相同，gag和myc结构域均被磷酸化。相比之下，MH2磷蛋白仅在gag结构域上被磷酸化。对部分转化缺陷型MC29缺失突变体的分析表明，缺失去除了v-myc特异性磷酸肽。磷酸氨基酸分析表明，这些缺失的磷酸肽在苏氨酸上被磷酸化。此外，恢复了转化能力的回复突变体重新获得了这些磷酸肽。这些研究将gag-myc蛋白的磷酸化与病毒的转化能力联系起来。
