Adachi K, Boyle S M, Sells B H
J Biol Chem. 1980 Jan 25;255(2):357-60.
The synthesis rate of ribosomal protein S1 was measured in Escherichia coli K-12 during the transitional period following a nutritional shift-up from acetate minimal to glucose/amino acids/nucleosides medium. The synthesis rate of S1 increased without a lag suggesting that the S1 gene is under stringent control and located very close to its promoter. The rate of S1 synthesis slowed between 7 and 15 min, and then increased to the postshift-up steady state rate. The postshift-up steady state rate was half the initial rate obtained between 0 and 7 min. The slow down in the synthesis rate between 7 and 15 min indicates that an unknown factor(s), in addition to guanosine 5'-diphosphate, 3'-diphosphate, participates in the regulation of S1 gene expression.
在从乙酸盐基本培养基营养转换到葡萄糖/氨基酸/核苷培养基后的过渡时期，对大肠杆菌K-12中核糖体蛋白S1的合成速率进行了测定。S1的合成速率无滞后地增加，这表明S1基因处于严格控制之下，并且其位置非常靠近其启动子。S1合成速率在7至15分钟之间减慢，然后增加到转换后的稳态速率。转换后的稳态速率是0至7分钟之间获得的初始速率的一半。7至15分钟之间合成速率的减慢表明，除了5'-二磷酸鸟苷、3'-二磷酸鸟苷之外，还有未知因素参与S1基因表达的调控。
