de Waal R M, Capel P J, Koene R A
J Immunol. 1980 Feb;124(2):719-23.
The enhancing and destructive effects of anti-H-2Kd and anti-H-2Dk antibodies were studied in B6AF1 recipients of B10.D2 or B10.BR skin allografts. The anti-H-2 antibodies were isolated from B6AF1 anti-B10.D2 and B6AF1 anti-B10.BR alloantiserum by adsorption to donor strain red blood cells and subsequent elution. Acute antibody-mediated rejection of B10.D2 or B10.BR skin grafts was readily induced by administration of anti-H-2K or H-2D antibodies, respectively, along with rabbit complement. Treatment of the B6AF1 recipients with the antibody eluates on day 0, 2, and 4 after transplantation resulted in specific and significant prolongation of graft survival. Both graft destruction and enhancement can therefore be mediated by antibodies directed against the same H-2 specificity.
在接受B10.D2或B10.BR皮肤同种异体移植的B6AF1受体中，研究了抗H-2Kd和抗H-2Dk抗体的增强和破坏作用。通过吸附到供体菌株红细胞上并随后洗脱，从B6AF1抗B10.D2和B6AF1抗B10.BR同种异体血清中分离出抗H-2抗体。分别给予抗H-2K或H-2D抗体以及兔补体，很容易诱导B10.D2或B10.BR皮肤移植物的急性抗体介导排斥反应。在移植后第0、2和4天用抗体洗脱液处理B6AF1受体，导致移植物存活时间特异性且显著延长。因此，针对相同H-2特异性的抗体既可以介导移植物破坏，也可以介导增强作用。
