Jansen J L, Koene R A, Kamp G J, Gerlag P G, Wijdeveld P A
J Immunol. 1975 May;114(5):1557-62.
B6AF1 anti-B10.D2 ascites fluid was fractionated by molecular sieving and cation exchange chromatography in an attempt to separate cytoxic and enhancing antibodies. Five fractions were obtained which showed no overlap on analysis with isoelectric focusing. Despite this complete physicochemial separation all fractions contained detectable amounts of 7S IgG1 and 7S IgG2. Furthermore, exhancement of B10.D2 skin grafts in B6AF1 recipients could be induced with all the fractions. Cytotoxic activity was also present in the fractions. This was not only shown in vitro by the cytolysis of B10.02 lymphoid cells in the presence of rabbit complement, but also by the ability of the different fractions to induce hyperacute destruction of B10.02 skin grafted onto B6AF1 mice after i.v. injection together with rabbit complement. Thus, we were unable to separate the alloantiserum in cytotoxic and enhancing fractions by physicochemical means.
通过分子筛和阳离子交换色谱法对B6AF1抗B10.D2腹水进行分级分离，试图分离出细胞毒性抗体和增强性抗体。获得了五个级分，经等电聚焦分析显示它们没有重叠。尽管进行了完全的物理化学分离，但所有级分均含有可检测量的7S IgG1和7S IgG2。此外，所有级分均可诱导B6AF1受体中B10.D2皮肤移植的增强。级分中也存在细胞毒性活性。这不仅在体外通过兔补体存在下B10.02淋巴细胞的细胞溶解得以显示，而且还通过不同级分在静脉注射兔补体后诱导移植到B6AF1小鼠上的B10.02皮肤超急性破坏的能力得以体现。因此，我们无法通过物理化学方法将同种异体抗血清分离为细胞毒性级分和增强性级分。
