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Antimicrob Agents Chemother. 1980 Jan;17(1):16-9. doi: 10.1128/AAC.17.1.16.
2
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Susceptibility to penicillins and cephalosporins in beta-lactamase producing strains of E. coli and relative amount of beta-lactamase produced from these strains.产β-内酰胺酶的大肠杆菌菌株对青霉素和头孢菌素的敏感性以及这些菌株产生的β-内酰胺酶的相对量。
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6
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Chromogenic redox assay for beta-lactamases yielding water-insoluble products. I. Kinetic behavior and redox chemistry.用于产生水不溶性产物的β-内酰胺酶的显色氧化还原测定法。I. 动力学行为和氧化还原化学
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1
Purification and characterization of macrolide 2'-phosphotransferase from a strain of Escherichia coli that is highly resistant to erythromycin.从一株对红霉素高度耐药的大肠杆菌中纯化和鉴定大环内酯2'-磷酸转移酶
Antimicrob Agents Chemother. 1989 Aug;33(8):1354-7. doi: 10.1128/AAC.33.8.1354.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Micro-iodometric assay for penicillinase.青霉素酶的微量碘量法测定
Biochem J. 1962 May;83(2):236-40. doi: 10.1042/bj0830236.
3
Iodometric assay of penicillinase.青霉素酶的碘量法测定
Nature. 1954 Nov 27;174(4439):1012-3. doi: 10.1038/1741012a0.
4
Rapid fixed-time assay for penicillinase.青霉素酶快速定时测定法
J Bacteriol. 1968 Apr;95(4):1493-4. doi: 10.1128/jb.95.4.1493-1494.1968.
5
The purification and properties of penicillin beta-lactamases mediated by transmissible R factors in Escherichia coli.大肠杆菌中由可传递R因子介导的青霉素β-内酰胺酶的纯化及特性
J Biochem. 1969 Jul;66(1):11-20. doi: 10.1093/oxfordjournals.jbchem.a129111.
6
The purification and properties of the -lactamase specified by the resistance factor R-1818 in Escherichia coli and Proteus mirabilis.大肠杆菌和奇异变形杆菌中由抗性因子R - 1818所指定的β - 内酰胺酶的纯化及特性
Biochem J. 1971 Jul;123(4):493-500. doi: 10.1042/bj1230493.
7
The beta-lactamases of gram-negative bacteria and their possible physiological role.革兰氏阴性菌的β-内酰胺酶及其可能的生理作用。
Adv Microb Physiol. 1973;9:31-88. doi: 10.1016/s0065-2911(08)60376-8.
8
A spectrophotometric assay of beta-lactamase action on penicillins.一种关于β-内酰胺酶对青霉素作用的分光光度测定法。
Biochem J. 1974 Jun;139(3):789-90. doi: 10.1042/bj1390789.
9
Measurement of beta-lactamase activity and rate of inactivation of penicillins by a pH-stat alkalimetric titration method.采用pH计碱量滴定法测定β-内酰胺酶活性及青霉素的失活速率。
J Pharm Sci. 1972 Oct;61(10):1594-8. doi: 10.1002/jps.2600611010.
10
Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate.一种使用显色头孢菌素底物检测β-内酰胺酶的新方法。
Antimicrob Agents Chemother. 1972 Apr;1(4):283-8. doi: 10.1128/AAC.1.4.283.

β-内酰胺酶的核磁共振光谱测定法

Nuclear magnetic resonance spectrometric assay of beta-lactamase.

作者信息

Kono M, O'Hara K, Shiomi Y

出版信息

Antimicrob Agents Chemother. 1980 Jan;17(1):16-9. doi: 10.1128/AAC.17.1.16.

DOI:10.1128/AAC.17.1.16
PMID:6986114
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC283719/
Abstract

Beta-Lactam antibiotics and the crude enzyme were mixed in deuterium oxide and placed in a nuclear magnetic resonance tube. The change of the nuclear magnetic resonance spectrum during the enzymatic reaction was then analyzed to determine beta-lactamase activity. By using beta-lactam antibiotics such as penicillins, cephalosporins, and cephamycins as substrates, a comparison of the beta-lactamase activities was made between the nuclear magnetic resonance spectrometric assay and the iodometric assay. There was a close correlation between these two methods.

摘要

将β-内酰胺抗生素与粗酶在重水中混合，然后置于核磁共振管中。接着分析酶促反应过程中核磁共振谱的变化，以确定β-内酰胺酶活性。以青霉素、头孢菌素和头霉素等β-内酰胺抗生素为底物，对核磁共振光谱测定法和碘量法的β-内酰胺酶活性进行了比较。这两种方法之间存在密切相关性。