β-内酰胺酶的核磁共振光谱测定法
Nuclear magnetic resonance spectrometric assay of beta-lactamase.
作者信息
Kono M, O'Hara K, Shiomi Y
出版信息
Antimicrob Agents Chemother. 1980 Jan;17(1):16-9. doi: 10.1128/AAC.17.1.16.
Abstract
Beta-Lactam antibiotics and the crude enzyme were mixed in deuterium oxide and placed in a nuclear magnetic resonance tube. The change of the nuclear magnetic resonance spectrum during the enzymatic reaction was then analyzed to determine beta-lactamase activity. By using beta-lactam antibiotics such as penicillins, cephalosporins, and cephamycins as substrates, a comparison of the beta-lactamase activities was made between the nuclear magnetic resonance spectrometric assay and the iodometric assay. There was a close correlation between these two methods.
摘要
将β-内酰胺抗生素与粗酶在重水中混合，然后置于核磁共振管中。接着分析酶促反应过程中核磁共振谱的变化，以确定β-内酰胺酶活性。以青霉素、头孢菌素和头霉素等β-内酰胺抗生素为底物，对核磁共振光谱测定法和碘量法的β-内酰胺酶活性进行了比较。这两种方法之间存在密切相关性。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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