流式细胞术数据的计算机化采集与分析。

Computerised acquisition and analysis of flow cytometric data.

作者信息

Blackledge G, Swindell R, Hodgson B W, Crowther D

出版信息

Int J Biomed Comput. 1980 Jan;11(1):41-52. doi: 10.1016/0020-7101(80)90005-7.

Abstract

An interface between a commercial flow cytometer (Ortho Cytofluorograf 4800) and a Desk Top Computer (H.P. 9845S) is described. Programs for data acquisition and analysis have been developed. These include cell DNA content analysis, 2-parameter (3D) histograms and sophisticated sub-population selection. Although cell sorting is not possible using the facility, the data acquired per cell is optimised and detailed analysis can be carried out. The system represents a flexible, easily controlled and economical method for storing and analysing flow cytometric data.

摘要

本文描述了一种商用流式细胞仪（Ortho Cytofluorograf 4800）与台式计算机（H.P. 9845S）之间的接口。已经开发了用于数据采集和分析的程序。这些程序包括细胞DNA含量分析、双参数（三维）直方图和复杂的亚群选择。虽然使用该设备无法进行细胞分选，但每个细胞获取的数据经过了优化，可以进行详细分析。该系统是一种灵活、易于控制且经济的存储和分析流式细胞术数据的方法。