Thrall R S, Peterson L B, Braley J F, Linehan J H, Dawson C A, Moore V L
J Lab Clin Med. 1980 Mar;95(3):343-50.
The systemic appearance of radioiodinated proteins (125I-OA and 131I-HSA) administered via the respiratory route was studied in normal rabbits and in rabbits with BCG-induced chronic granulomatous pulmonary inflammation. The proteins were administered by i.t. injection into intact rabbits and into rabbits with tracheal cannulas or as an aerosol into isolated perfused lungs. The results showed that radioactivity appeared in the circulation as two fractions, one that was precipitables with 5% TCA and therefore protein-bound and one that was soluble in TCA. In both intact and tracheostomized animals, significantly more protein-;ound radioactive iodine was detected in the circulation of BCG-treated animals than in normal animals as early as 15 min after i.t. injection, and the differences persisted from 2 to 4 hr. However, in the isolated perfused lung, in which the only route for protein uptake into the circulation was the alveolocapillary barrier, only minimal differences in blood protein levels were observed as compared to normal BCG-inflamed lungs. This study suggests that chronic pulmonary inflammation promotes the absorption of i.t.-injected protein into the circulation, and that the route of enhanced uptake into blood is not the alveolocapillary membrane.
通过呼吸道途径给予放射性碘化蛋白（¹²⁵I - OA和¹³¹I - HSA）后，在正常兔和卡介苗诱导的慢性肉芽肿性肺炎兔中研究了其全身表现。通过经气管内注射将蛋白质给予完整的兔子和有气管插管的兔子，或将其制成气雾剂给予离体灌注肺。结果表明，放射性以两种组分出现在循环中，一种可被5%三氯乙酸沉淀，因此是与蛋白结合的，另一种可溶于三氯乙酸。在完整动物和气管切开动物中，早在经气管内注射后15分钟，在卡介苗处理动物的循环中检测到的与蛋白结合的放射性碘就比正常动物显著更多，并且这种差异在2至4小时内持续存在。然而，在离体灌注肺中，蛋白质进入循环的唯一途径是肺泡毛细血管屏障，与正常卡介苗感染的肺相比，仅观察到血液蛋白水平有极小差异。这项研究表明，慢性肺部炎症促进经气管内注射的蛋白质吸收进入循环，并且增强进入血液的途径不是肺泡毛细血管膜。
