Braley J F, Dawson C A, Moore V L
J Immunol. 1978 Sep;121(3):926-9.
In previous studies with isolated perfused rabbit lungs, we observed that inhaled human serum albumin (HSA) or ovalbumin (OA) entered the pulmonary circulation antigenically intact. The inhaled proteins were also metabolized in the lung. Immunization reduced the amount of intact protein and increased the amount of metabolites absorbed. In the present study, we have begun to characterize the immune mechanisms responsible for reduced antigen absorption. A humoral immune mechanism appeared to be involved because the phenomenon could be passively transferred to normal animals by administering immune serum either 18 hr or immediately before antigen inhalation. The reduction was also observed when lungs from immunized rabbits were perfused with normal rabbit blood, indicating that antibodies in both lung and blood may be involved. Experiments, in which lungs from immunized rabbits were simultaneously insufflated with the immunizing antigen and with a nonspecific protein, demonstrated that the block against antigen absorption was specific for the immunizing antigen and was not due to some antigen-induced nonspecific changes in lung physiology.
在先前对离体灌注兔肺的研究中，我们观察到吸入的人血清白蛋白（HSA）或卵清蛋白（OA）以抗原完整的形式进入肺循环。吸入的蛋白质也在肺中代谢。免疫作用减少了完整蛋白质的量，并增加了吸收的代谢物的量。在本研究中，我们已开始对负责减少抗原吸收的免疫机制进行表征。似乎涉及一种体液免疫机制，因为该现象可通过在抗原吸入前18小时或立即给予免疫血清而被动转移至正常动物。当用正常兔血灌注免疫兔的肺时也观察到了减少，这表明肺和血液中的抗体可能都参与其中。用免疫抗原和非特异性蛋白质同时向免疫兔的肺吹气的实验表明，对抗抗原吸收的阻断对免疫抗原具有特异性，并非由于抗原诱导的肺生理学的某些非特异性变化。
