Nakashima T, Makishima K, Hiroto I
Ann Otol Rhinol Laryngol. 1980 Jan-Feb;89(1 Pt 1):24-8. doi: 10.1177/000348948008900107.
We succeeded in deriving a long-term tissue culture cell line from human maxillary sinus carcinoma. This cell line, designated as MC, was passaged 100 times in vitro over a period of 18 months. The cells are globular in shape, grow as single cells in the culture medium, and the mean population doubling time is about 12 hours. The plating efficiency rate in soft agar is 78% and chromosomal analysis revealed the modal chromosome number to be between 47 and 51. These MC cells were transplanted into five nude mice, all of which developed a tumor after a latency period of 5 to 8 days and died within 39 days. Complete autopsy of all mice revealed no metastasis. Histopathological findings of the original and the transplanted tumor tissues showed a remarkable similarity.
我们成功地从人上颌窦癌中获得了一种长期组织培养细胞系。该细胞系命名为MC，在18个月的时间里在体外传代了100次。细胞呈球形，在培养基中以单细胞形式生长，平均群体倍增时间约为12小时。软琼脂中的接种效率率为78%，染色体分析显示众数染色体数在47至51之间。将这些MC细胞移植到五只裸鼠体内，所有裸鼠在5至8天的潜伏期后都长出了肿瘤，并在39天内死亡。对所有小鼠进行的完整尸检未发现转移。原始肿瘤组织和移植肿瘤组织的组织病理学结果显示出显著的相似性。
