患有乳腺炎的奶牛外周血中B淋巴细胞水平降低。
Depression of B-lymphocyte levels in the peripheral blood of cows with mastitis.
作者信息
Yang T J, Jantzen-Abo P A, Williams L F
出版信息
Infect Immun. 1980 Jan;27(1):90-3. doi: 10.1128/iai.27.1.90-93.1980.
The levels of B-lymphocytes in the peripheral blood of normal and mastitic cows were evaluated by fluorescent-antibody and erythrocyte-antibody-complement-rosetting techniques. Normal cows (N = 8) had 26.9 +/- 4.4% surface membrane immunoglobulin-positive lymphocytes, whereas mastitic cows (N = 6) had only 16.3 +/- 3.6% similar cells among lymphocyte preparations purified by the Ficoll-Paque density gradient separation and carbonyl iron phagocytosis methods. Studies on smiliar lymphocyte preparations by erythrocyte-antibody-complement-rosetting techniques showed that normal cows (N = 12) had 33.5 +/- 7.1% erythrocyte-antibody-complement-rosetting lymphocytes, whereas mastitic cows (N = 6) had 20.5 +/- 6.0% similar cells.
通过荧光抗体和红细胞抗体补体玫瑰花结技术评估正常奶牛和患乳腺炎奶牛外周血中B淋巴细胞的水平。正常奶牛（N = 8）有26.9±4.4%的表面膜免疫球蛋白阳性淋巴细胞，而通过Ficoll-Paque密度梯度分离和羰基铁吞噬法纯化的淋巴细胞制剂中，患乳腺炎奶牛（N = 6）只有16.3±3.6%的类似细胞。通过红细胞抗体补体玫瑰花结技术对类似淋巴细胞制剂的研究表明，正常奶牛（N = 12）有33.5±7.1%的红细胞抗体补体玫瑰花结淋巴细胞，而患乳腺炎奶牛（N = 6）有20.5±6.0%的类似细胞。
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