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粪便冷增菌法对从患者中分离小肠结肠炎耶尔森菌3型和9型血清型无价值。

Nonvalue of cold enrichment of stools for isolation of Yersinia enterocolitica serotypes 3 and 9 from patients.

作者信息

Van Noyen R, Vandepitte J, Wauters G

出版信息

J Clin Microbiol. 1980 Feb;11(2):127-31. doi: 10.1128/jcm.11.2.127-131.1980.

Abstract

During an 8-year period 14,092 fecal specimens and 1,428 excised appendixes were examined for the presence of Yersinia enterocolitica, with different combinations of direct plating media and enrichment techniques. The combination of direct plating on SS agar and 2 days of enrichment in a modified Rappaport broth at room temperature resulted in the isolation of 100% of serotype 3 and 9 strains. Such strains were recovered from 3.7% of our fecal specimens. Cold enrichment in phosphate buffer further increased the isolation rate, but the additional isolates all belonged to biotype 1. Evidence is presented that biotype 1 strains, at least in Belgium, are not pathogenic for humans. There was a significant affinity of serotype 9 strains for patients suffering from an "appendicular syndrome."

摘要

在8年期间,采用不同的直接接种培养基和增菌技术组合,对14092份粪便标本和1428份切除的阑尾进行了小肠结肠炎耶尔森菌检测。直接接种于SS琼脂并在室温下于改良Rappaport肉汤中增菌2天的组合,分离出了100%的3型和9型菌株。此类菌株从我们3.7%的粪便标本中检出。在磷酸盐缓冲液中进行冷增菌进一步提高了分离率,但额外分离出的菌株均属于生物型1。有证据表明,至少在比利时,生物型1菌株对人类无致病性。9型菌株对患有“阑尾综合征”的患者有显著亲和力。

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