Pai C H, Sorger S, Lafleur L, Lackman L, Marks M I
J Clin Microbiol. 1979 Jun;9(6):712-5. doi: 10.1128/jcm.9.6.712-715.1979.
Stool specimens from children with gastroenteritis and their household contacts were cultured for Yersinia enterocolitica by direct plating onto routine laboratory media. These stools were also inoculated into phosphate-buffered saline and subcultured to the same media after 1 day or 3 weeks of incubation at 4 degrees C. Y. enterocolitica was isolated from 174 index cases and 34 household contacts. One hundred eighty-one isolates were of serotype O:3, and the remaining 21 belonged to other serotypes. Eighty-one percent (147/181) of O:3 isolates were recovered by direct plating, and 6.1% (11/181) and 13% (23/181) were recovered by 1-day and 3-week cold enrichment, respectively. For other serotypes, 26% (7/27), 0%, and 74% (20/27) were isolated by direct plating, 1-day cold enrichment, and 3-week cold enrichment, respectively. The efficacy of the cold enrichment for the patients were still symptomatic, 94 and 6% of Y. enterocolitica were identified by direct plating and cold enrichment, respectively. Isolation rates were 66% by direct plating and 34% by cold enrichment when stools were obtained from asymptomatic carriers or from those convalescing from Y. enterocolitica gastroenteritis. These results indicate that the cold enrichment methods increase the sensitivity of Y. entercolitica culture methods considerably in convalescent and asymptomatic subjects but only minimally in patients with diarrhea caused by serotype O:3.
将患有肠胃炎儿童及其家庭接触者的粪便标本直接接种到常规实验室培养基上，培养小肠结肠炎耶尔森菌。这些粪便标本还接种到磷酸盐缓冲盐水中，并在4℃下孵育1天或3周后，再接种到相同的培养基上进行传代培养。从174例索引病例和34名家庭接触者中分离出小肠结肠炎耶尔森菌。181株分离株中，181株为O:3血清型，其余21株属于其他血清型。O:3分离株中，81%（147/181）通过直接接种培养获得，1天和3周冷增菌法分别分离出6.1%（11/181）和13%（23/181）。对于其他血清型，直接接种培养、1天冷增菌法和3周冷增菌法分别分离出26%（7/27）、0%和74%（20/27）。对于仍有症状的患者，冷增菌法的效果方面，直接接种培养和冷增菌法分别鉴定出94%和6%的小肠结肠炎耶尔森菌。当从无症状携带者或小肠结肠炎耶尔森菌肠胃炎康复者获取粪便时，直接接种培养的分离率为66%，冷增菌法的分离率为34%。这些结果表明，冷增菌法可显著提高恢复期和无症状受试者小肠结肠炎耶尔森菌培养方法的敏感性，但对O:3血清型腹泻患者的敏感性提高幅度很小。
