Bascomb S, Spencer R C
J Clin Pathol. 1980 Jan;33(1):36-46. doi: 10.1136/jcp.33.1.36.
Automated methods for measuring enzyme activities of bacterial suspensions in saline are described. The methods were applied to bacteria cultured from urine specimens, and specific enzyme profiles characteristic for Escherichia coli, Klebsiella sp, Proteus sp, and Pseudomonas sp were established. Identification of 294 freshly isolated strains by automated and conventional methods were compared. Results from automated identification based on eight enzyme tests and assay of protein content, all performed on a bacterial suspension made from one colony in 1 ml of saline, agreed 100% with those obtained by conventional methods. Identification was achieved in 6 hours.
本文描述了用于测量盐溶液中细菌悬液酶活性的自动化方法。这些方法应用于从尿液标本中培养出的细菌，并建立了大肠杆菌、克雷伯菌属、变形杆菌属和假单胞菌属特有的特定酶谱。比较了通过自动化方法和传统方法对294株新分离菌株的鉴定结果。基于八项酶测试和蛋白质含量测定的自动化鉴定结果，所有测试均在1毫升盐溶液中一个菌落制成的细菌悬液上进行，与传统方法获得的结果100%一致。6小时内完成了鉴定。
