Jong E C, Henderson W R, Klebanoff S J
J Immunol. 1980 Mar;124(3):1378-82.
A partially purified preparation of guinea pig eosinophil peroxidase was found to be bactericidal when combined with H2O2 and either iodide, bromide, chloride, or thiocyanate ions. The EPO-H2O2-halide bactericidal system had an acid pH optimum and was inhibited by the proteins albumin and gelatin and by the hemeprotein inhibitors azide, cyanide, and aminotriazole. When the EPO concentration of the reaction mixture was lowered, the bactericidal effect at pH 7.0 was lost first with chloride, then with bromide, and finally with iodide as the halide. Activity with physiologic concentrations of chloride was favored by a relatively high EPO level, a decrease in pH below neutrality and an absence of extraneous protein. These findings are discussed in relation to the potential role of the peroxidase system in the intracellular and extracellular toxic reactions of eosinophils.
当豚鼠嗜酸性粒细胞过氧化物酶的部分纯化制剂与过氧化氢以及碘离子、溴离子、氯离子或硫氰酸根离子结合时，发现其具有杀菌作用。EPO-H₂O₂-卤化物杀菌系统的最适pH为酸性，并且受到蛋白质白蛋白和明胶以及血红素蛋白抑制剂叠氮化物、氰化物和氨基三唑的抑制。当反应混合物中EPO浓度降低时，在pH 7.0条件下，随着卤化物依次为氯离子、溴离子和碘离子，杀菌作用首先丧失。相对较高的EPO水平、pH降至中性以下以及不存在外源蛋白质有利于生理浓度氯离子的活性。本文就过氧化物酶系统在嗜酸性粒细胞细胞内和细胞外毒性反应中的潜在作用对这些发现进行了讨论。
