完整动物中的基因转移。
Gene transfer in intact animals.
作者信息
Cline M J, Stang H, Mercola K, Morse L, Ruprecht R, Brown J, Salser W
出版信息
Nature. 1980 Apr 3;284(5755):422-5. doi: 10.1038/284422a0.
DOI:10.1038/284422a0
PMID:6987538
Abstract
Resistance to methotrexate was induced in bone marrow cells of mice by transformation in vitro with DNA from a drug-resistant cell line. Transformed cells were injected in vivo and haematopoietic cells expressing resistance were selected by drug treatment of recipients. Transformed cells had elevated levels of dihydrofolate reductase and demonstrated a proliferative advantage over untransformed cells, indicating successful gene transfer.
摘要
通过用来自耐药细胞系的DNA进行体外转化，在小鼠骨髓细胞中诱导出对甲氨蝶呤的抗性。将转化细胞注射到体内，并通过对受体进行药物处理来选择表达抗性的造血细胞。转化细胞的二氢叶酸还原酶水平升高，并且显示出比未转化细胞具有增殖优势，表明基因转移成功。
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