Debenham P G, Pongs O, Travers A A
Proc Natl Acad Sci U S A. 1980 Feb;77(2):870-4. doi: 10.1073/pnas.77.2.870.
Escherichia coli fMet-tRNAfMet alters the pattern of promoter selection of E. coli RNA polymerase (RNA nucleotidyltransferase, nucleosidetriphosphate:RNA nucleotidyltransferase; EC 2.7.7.6), affecting RNA synthesis from the rRNA, suIII+tRNA, and lac promoters in different ways. The in vitro synthesis of the stable RNA species is selectively decreased, whereas that of lac RNA from both the wild-type and mutant UV5 promoters is selectively increased at high ionic strength. The functional effect of fMet-tRNAfMet resembles that of the nucleotide guanosine 3'-diphosphate 5'-diphosphate (ppGpp). This nucleotide competes with the binding of fMet-tRnafMet to RNA polymerase.
大肠杆菌甲硫氨酰 - tRNAfMet改变了大肠杆菌RNA聚合酶（RNA核苷酸转移酶，核苷三磷酸：RNA核苷酸转移酶；EC 2.7.7.6）的启动子选择模式，以不同方式影响来自rRNA、suIII + tRNA和lac启动子的RNA合成。稳定RNA种类的体外合成选择性降低，而在高离子强度下，来自野生型和突变型UV5启动子的lac RNA的合成选择性增加。甲硫氨酰 - tRNAfMet的功能作用类似于核苷酸鸟苷3'-二磷酸5'-二磷酸（ppGpp）。这种核苷酸与甲硫氨酰 - tRNAfMet与RNA聚合酶的结合相互竞争。
