Crane M S, Dvorak J A
Science. 1980 Apr 11;208(4440):194-6. doi: 10.1126/science.6987737.
Epimastigotes, the invertebrate host stage of Trypanosoma cruzi, the protozoan parasite causing Chagas' disease in man, were fused with vertebrate cells by using polyethylene glycol. Hybrid cells were selected on the basis of T. cruzi DNA complementation of biochemical deficiencies in the vertebrate cells. Some clones of the hybrid cells expressed T. cruzi-specific antigen. It might be possible to use selected antigens obtained from the hybrids as vaccines for immunodiagnosis or for elucidation of the pathogenesis of Chagas' disease.
克氏锥虫（Trypanosoma cruzi）是一种在人类中引发恰加斯病的原生动物寄生虫，其无脊椎动物宿主阶段的上鞭毛体，通过聚乙二醇与脊椎动物细胞融合。杂交细胞是根据克氏锥虫DNA对脊椎动物细胞生化缺陷的互补作用来选择的。一些杂交细胞克隆表达了克氏锥虫特异性抗原。有可能将从杂交细胞中获得的选定抗原用作免疫诊断疫苗或用于阐明恰加斯病的发病机制。
