Hall J G
Ciba Found Symp. 1980;71:197-209. doi: 10.1002/9780470720547.ch11.
A characteristic feature of the induction of cell-mediated delayed hypersensitivity reactions by chemicals such as oxazolone is the enlargement of lymphocyte traffic areas in the paracortices of regional lymph nodes. In sheep oxazolone is a powerful immunogen but the cellular changes in lymph efferent from nodes draining areas of oxazolone-painted skin do not differ significantly from responses to conventional antigens. Specific complement-binding antibodies appear in the plasma of sensitized sheep, which respond to secondary challenges with an immediate Arthus reaction. In studies of peripheral lymph from areas of skin painted with oxazolone the number of mononuclear cells in the lymph increased 10--50-fold two days or so after skin painting. Most of these cells were small lymphocytes lacking surface immunoglobulin (presumptive 'T' cells). This big increase in lymphocyte traffic through the skin may be a consequence of the binding to local structural proteins of myriads of oxazolone epitopes. If so, and bearing in mind the large doses of immunogen used in experiments on mice, it is easy to envisage how the traffic areas of lymph nodes expand and become congested with lymphocytes after being flooded with a highly immunogenic and reactive chemical like oxazolone. Whether this is relevant to the induction of cell-mediated immunity is unknown.
诸如恶唑酮之类的化学物质诱导细胞介导的迟发型超敏反应的一个特征是区域淋巴结副皮质区淋巴细胞流通区域的扩大。在绵羊中，恶唑酮是一种强大的免疫原，但从涂抹恶唑酮皮肤区域引流的淋巴结输出淋巴中的细胞变化与对传统抗原的反应并无显著差异。致敏绵羊的血浆中会出现特异性补体结合抗体，它们对再次攻击会产生即刻的阿瑟斯反应。在对涂抹恶唑酮皮肤区域的外周淋巴进行研究时，皮肤涂抹后两天左右，淋巴中单核细胞的数量增加了10至50倍。这些细胞大多数是缺乏表面免疫球蛋白的小淋巴细胞（推测为“T”细胞）。淋巴细胞通过皮肤的大量增加可能是无数恶唑酮表位与局部结构蛋白结合的结果。如果是这样，并且考虑到在小鼠实验中使用的大剂量免疫原，就很容易设想在用像恶唑酮这样具有高度免疫原性和反应性的化学物质充斥后，淋巴结的流通区域如何扩大并被淋巴细胞充斥。这是否与细胞介导免疫的诱导有关尚不清楚。
