Hoffman A G, Kuksis A
Experientia. 1980 Feb 15;36(2):202-4. doi: 10.1007/BF01953732.
Strains of presumptive epithelial and fibroblast cells were prepared from the jejunal mucosa of axenic rats. Cells were cultured on collagen gels, in highly enriched media supplemented with homologous sera and hormones, and were maintained for more than 7 weeks.
从无菌大鼠的空肠黏膜制备出推定的上皮细胞和成纤维细胞系。细胞在胶原凝胶上培养,置于添加了同源血清和激素的高度富集培养基中,并维持培养超过7周。