原发性混合淋巴细胞反应中的淋巴因子产生。I. 反应细胞和刺激细胞的特征。

Lymphokine production in primary mixed lymphocyte reactions. I. Characteristics of responding and stimulating cells.

作者信息

Landolfo S, Di Matteo C, Capusso A, Negro-Ponzi A, Forni G

出版信息

Exp Cell Biol. 1980;48(3):180-90. doi: 10.1159/000162986.

Abstract

The characteristics of the responder and stimulating cells involved in migration inhibition factor (MIF) production in primary 'one-way' mouse mixed lymphocyte reactions (MLR) were analyzed by using an indirect agarose droplet assay. T-lymphocytes are mainly responsible for MIF release, as shown by pretreatment with anti-Thy 1.2 serum plus complement or purification over a nylon wool column. On the other hand, macrophages and B-lymphocytes appear to be optimal stimulating cells. T-lymphocytes as stimulating cells induce MIF release, but to a much lesser degree than macrophages and B-lymphocytes. The kinetics of MIF production in MLR is related to the kind of stimulating cells employed. Lastly, the ability to release MIF is already present in the spleen of 1- to 2-week-old mice, lasts until 20 weeks of age and declines to undetectable levels at 50 weeks of age.

摘要

通过间接琼脂糖滴试验分析了参与原发性“单向”小鼠混合淋巴细胞反应（MLR）中迁移抑制因子（MIF）产生的应答细胞和刺激细胞的特征。抗Thy 1.2血清加补体预处理或通过尼龙毛柱纯化表明，T淋巴细胞主要负责MIF的释放。另一方面，巨噬细胞和B淋巴细胞似乎是最佳刺激细胞。T淋巴细胞作为刺激细胞可诱导MIF释放，但程度远低于巨噬细胞和B淋巴细胞。MLR中MIF产生的动力学与所用刺激细胞的种类有关。最后，释放MIF的能力在1至2周龄小鼠的脾脏中就已存在，持续到20周龄，并在50周龄时降至无法检测的水平。