Fjeld J
J Immunol Methods. 1980;33(3):213-20. doi: 10.1016/0022-1759(80)90208-2.
A flow cytophotometry method has been evaluated for the enumeration of rat lymphocyte subpopulations in lymph and lymph nodes. The cells were prepared by incubating them with complement and anti-Ig or anti-T antiserum, followed by trypan blue staining. Cytophotometry was used for automatic registration of dead and viable cells. Parallel viability counts were done in a microscope. In addition the cytotoxic activity of the anti-Ig antiserum was compared with its surface binding capacity in a direct immunofluorescence assay. Correlation was found between the automatic and microscopical registrations.
已对一种流式细胞光度法进行了评估，用于计数大鼠淋巴液和淋巴结中的淋巴细胞亚群。通过将细胞与补体、抗Ig或抗T抗血清孵育，然后进行台盼蓝染色来制备细胞。细胞光度法用于自动记录死细胞和活细胞。在显微镜下进行平行的活力计数。此外，在直接免疫荧光试验中比较了抗Ig抗血清的细胞毒性活性与其表面结合能力。发现自动记录和显微镜记录之间存在相关性。
