Close T J, Shanmugam K T
J Gen Microbiol. 1980 Feb;116(2):501-10. doi: 10.1099/00221287-116-2-501.
Genetic and reversion analyses of a thermosensitive pleiotropic mutant strain of Klebsiella pneumoniae with defects in nitrogen fixation and nitrogen metabolism have shown that the pleiotropie behaviour of mutant is due to a single mutation in a gene designated nim. This gene is contransducible with trp at a frequency of about 30% (using bacteriophage P1) and with cys at a frequency of about 14%. The gene order is cys, trp, nim. The defect in the nim mutant is complemented by the E. coli F' element, F'148.
对肺炎克雷伯菌一株固氮和氮代谢有缺陷的温度敏感多效突变株进行的遗传和回复分析表明，该突变株的多效行为是由于一个名为nim的基因发生了单一突变。该基因与trp以约30%的频率共转导（使用噬菌体P1），与cys以约14%的频率共转导。基因顺序为cys、trp、nim。nim突变体的缺陷可被大肠杆菌F'因子F'148互补。
