St John R T, Johnston H M, Seidman C, Garfinkel D, Gordon J K, Shah V K, Brill W J
J Bacteriol. 1975 Mar;121(3):759-65. doi: 10.1128/jb.121.3.759-765.1975.
Selected mutant strains of Klebsiella pneumoniae that are unable to fix nitrogen have been characterized according to nitrogenase component activity as well as antigenic cross-reacting material. The lesions in these strains have been mapped by transduction, and the results indicate that there are at least five genes specifically responsible for nitrogen fixation in vivo. Besides genes that specify the structure of the two nitrogenase components, there is a gene for a factor that is required for component I activity and a gene that codes for a factor possibly involved in electron transport to component II. A mutation in another site does not allow the organism to produce either of the nitrogenase components. All of these genes are co-transducible with the gene that specifics the structure of histidinol dehydrogenase.
已根据固氮酶成分活性以及抗原交叉反应物质对所选不能固氮的肺炎克雷伯菌突变株进行了表征。这些菌株中的损伤已通过转导进行定位，结果表明体内至少有五个基因专门负责固氮。除了指定两种固氮酶成分结构的基因外，还有一个负责成分I活性所需因子的基因以及一个编码可能参与向成分II进行电子传递的因子的基因。另一位点的突变使该生物体无法产生任何一种固氮酶成分。所有这些基因都与指定组氨醇脱氢酶结构的基因共转导。
