Chernyakhovskaya I Y, Nagurskaya E V, Shaposhnikova G B, Prigozhina T B, Fontalin L N
Transplantation. 1980 May;29(5):409-12. doi: 10.1097/00007890-198005000-00013.
A new method of induction of tolerance to allogeneic and to xenogeneic cells is presented. It includes thymectomy of adult mice followed 1 month later by the injection of 1 X 10(8) spleen cells i.v. and i.p. administration of 200 mg of cyclophosphamide per kg 1 day after cells. This method induced prolonged survival of heterotopically transplanted neonatal C57BL/6 murine heart grafts (more than 8 months) and of August rat heart grafts (more than 2 months) in CBA mice. Tolerance to allo- or xenoantigens was formed at the cell level. Experimental animals did not produce allo- or xenohemagglutinins after graft implantation. Spleen cells of mice with surviving C57BL/6 heart grafts did not respond to C57BL/6 cells in mixed lymphocyte culture (MLC) reaction. Lymphoid cell chimerism was not observed in animals tolerant to alloantigens.
本文提出了一种诱导对同种异体细胞和异种异体细胞产生耐受性的新方法。该方法包括对成年小鼠进行胸腺切除术，1个月后静脉注射1×10⁸个脾细胞，并在注射细胞1天后腹腔注射每千克200毫克环磷酰胺。此方法可使CBA小鼠体内异位移植的新生C57BL/6小鼠心脏移植物（存活超过8个月）和奥古斯特大鼠心脏移植物（存活超过2个月）长期存活。对同种或异种抗原的耐受性在细胞水平形成。移植后，实验动物不产生同种或异种血细胞凝集素。具有存活C57BL/6心脏移植物的小鼠的脾细胞在混合淋巴细胞培养（MLC）反应中对C57BL/6细胞无反应。在对同种抗原产生耐受的动物中未观察到淋巴细胞嵌合体。
