Iserentant D, Fiers W
Gene. 1980 Apr;9(1-2):1-12. doi: 10.1016/0378-1119(80)90163-8.
A series of recombinant plasmids, containing the cro gene of phage lambda, exhibit strikingly different levels of expression depending apparently only on the nucleotide sequence of the untranslated 5' mRNA (Roberts et al., 1979). We postulate that initiation of translation involves interaction between an activated 30S ribosomal subunit and the 5'-terminal region of a messenger RNA already folded in a specific secondary structure. The observed variation in cro synthesis can then adequately be explained by secondary structure models which were derived for the different mRNAs. To maximize expression, it appears necessary that the initiation codon and, although less important, the ribosome interaction site are accessible.
一系列含有噬菌体λ cro基因的重组质粒，其表达水平明显不同，这显然仅取决于未翻译的5' mRNA的核苷酸序列（罗伯茨等人，1979年）。我们推测，翻译起始涉及活化的30S核糖体亚基与已折叠成特定二级结构的信使RNA的5'末端区域之间的相互作用。然后，可以通过针对不同mRNA推导的二级结构模型充分解释观察到的cro合成变化。为了使表达最大化，起始密码子以及虽然不太重要但核糖体相互作用位点似乎必须是可接近的。
