Starcher B C, Glauber J G, Madaras J G
J Nutr. 1980 Jul;110(7):1391-7. doi: 10.1093/jn/110.7.1391.
This study examined the relationship between zinc absorption and metallothionein. Mice injected intraperitoneally with ZnCl2 (2 mmoles) were found within 18 hours to have increased levels of intestinal metallothionein but an apparent decrease in 65Zn absorption. Induction of metallothionein with lower levels of ZnCl2 (0.2 or 0.5 mumoles) resulted in an apparent increase in 65Zn absorption. Isotope dilution experiments showed that intraperitoneal injections of 2 mumoles of ZnCl2 had resulted in a 500-fold dilution of the available 65Zn pool. Mild stress of the animals was shown to increase both 65Zn absorption and intestinal metallothionein. Actinomycin D administered 4 hours prior to ZnCl2 or stress, prevented the induction of metallothionein and obliterated the increase in 65Zn absorption. These results indicate that zinc absorption is directly proportional to intestinal metallothionein levels and imply a significant role for metallothionein in zinc absorption.
本研究检测了锌吸收与金属硫蛋白之间的关系。腹腔注射氯化锌（2毫摩尔）的小鼠在18小时内肠道金属硫蛋白水平升高，但65锌吸收明显下降。用较低水平的氯化锌（0.2或0.5微摩尔）诱导金属硫蛋白导致65锌吸收明显增加。同位素稀释实验表明，腹腔注射2微摩尔氯化锌导致可用65锌池稀释500倍。对动物施加轻度应激可增加65锌吸收和肠道金属硫蛋白。在氯化锌或应激前4小时给予放线菌素D，可阻止金属硫蛋白的诱导并消除65锌吸收的增加。这些结果表明，锌吸收与肠道金属硫蛋白水平成正比，并暗示金属硫蛋白在锌吸收中起重要作用。
