Nolan C M, LaBorde E A, Howell R T, Robbins J B
J Med Microbiol. 1980 May;13(2):373-7. doi: 10.1099/00222615-13-2-373.
An antiserum-agar technique was evaluated as a method for detecting Salmonella typhi in faeces. Thirty-one laboratory strains of S. typhi produced immunoprecipitate haloes during overnight growth on SS agar and blood-agar-base infusion agar (BAB) containing donkey antiserum to a vaccine strain of S. typhi. Other salmonella species sharing O serogroup antigens with S. typhi also produced haloes when streaked in pure culture on SS-antiserum agar but not on BAB-antiserum agar. One hundred and forty-one consecutive faecal specimens were cultured on SS-antiserum agar. Results with this method were concordant with those of established isolation techniques on specimens from six of seven suspected carriers of S. typhi. Ten other salmonellas were isolated from the faecal specimens but only S. javiana, like S. typhi a serogroup-D organism, yielded false-positive haloes on antiserum agar. The antiserum-agar technique offers promise as a means of screening for S. typhi in faecal cultures.
一种抗血清-琼脂技术被评估为检测粪便中伤寒沙门氏菌的方法。31株伤寒沙门氏菌实验室菌株在含有针对伤寒沙门氏菌疫苗株的驴抗血清的SS琼脂和血琼脂基础肉汤琼脂（BAB）上过夜培养期间产生了免疫沉淀晕圈。与伤寒沙门氏菌共享O血清群抗原的其他沙门氏菌物种在纯培养物中划线接种于SS-抗血清琼脂上时也产生晕圈，但在BAB-抗血清琼脂上则不产生。141份连续粪便标本在SS-抗血清琼脂上培养。该方法的结果与来自7名疑似伤寒沙门氏菌携带者中6人的标本的既定分离技术结果一致。从粪便标本中分离出了另外10种沙门氏菌，但只有哈维亚纳沙门氏菌，与伤寒沙门氏菌一样是D血清群的生物体，在抗血清琼脂上产生假阳性晕圈。抗血清-琼脂技术有望作为粪便培养物中伤寒沙门氏菌筛查的一种手段。
