[Autograft of pancreatic tissue in the spleen of pancreatectomized dogs].

The intrasplenic autotransplant of pancreatic fragments in dogs subjected to total pancreatectomy can prevent diabetes as it maintains normoglycaemic condition and a normal insulinaemic response to i.v. infusion of glucose. By adopting a pancreatic tissue preparation technique which provides for a very short collagenasis digestion time (4 minutes), B cells took and normal glucidic metabolism was maintained.