[从大肠杆菌中制备性分离多核糖体并检测其性质]
[Preparative isolation of polyribosomes from E. coli, and examination of their properties].
作者信息
Ludriks M R
出版信息
Prikl Biokhim Mikrobiol. 1980 Mar-Apr;16(2):238-44.
PMID:6992136
Abstract
The paper describes a preparative method to produce polyribosomes from E. coli via their precipitation in a step-by-step sucrose gradient in the Ti-15 zonal rotor. Polyribosomes had the ability to translate mRNA in vitro. Polyribosomal proteins were isolated by the method of two-dimensional electrophoresis. The elongation factor EF-G was shown to occur in the polyribosomes.
摘要
该论文描述了一种制备方法，即通过在Ti-15区带转子中逐步蔗糖梯度沉淀从大肠杆菌中制备多核糖体。多核糖体具有在体外翻译mRNA的能力。通过二维电泳方法分离多核糖体蛋白。结果表明延伸因子EF-G存在于多核糖体中。
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