[Preparative isolation of polyribosomes from E. coli, and examination of their properties].

The paper describes a preparative method to produce polyribosomes from E. coli via their precipitation in a step-by-step sucrose gradient in the Ti-15 zonal rotor. Polyribosomes had the ability to translate mRNA in vitro. Polyribosomal proteins were isolated by the method of two-dimensional electrophoresis. The elongation factor EF-G was shown to occur in the polyribosomes.