猪主动脉前列环素合酶对前列腺素内过氧化物的转化作用。
Conversions of prostaglandin endoperoxides by prostacyclin synthase from pig aorta.
作者信息
Wlodawer P, Hammarström S
出版信息
Prostaglandins. 1980 Jun;19(6):969-76. doi: 10.1016/0090-6980(80)90129-x.
Partially purified prostacyclin synthase from pig aorta converted the prostaglandin (PG) endoperoxide PGH2 to prostacyclin (PGI2), and PGH1 to 12-hydroxy-8,10-heptadecadienoic acid (HHD). Both reactions were inhibited by 15-hydroperoxy-5,8,11,13-eicosatetraenoic acid (15-HP) in a dose-dependent rashion. However, the reactions PGH2 leads to PGI2 and PGH1 leads to HHD appeared to differ: substrate availability was rate limiting in the latter reaction, while the enzyme became rapidly saturated witth PGH2 and a steady rate of prostacyclin formation was observed at higher substrate levels.
从猪主动脉中部分纯化得到的前列环素合酶可将前列腺素（PG）内过氧化物PGH2转化为前列环素（PGI2），并将PGH1转化为12-羟基-8,10-十七碳二烯酸（HHD）。这两种反应均受到15-氢过氧-5,8,11,13-二十碳四烯酸（15-HP）的剂量依赖性抑制。然而，PGH2转化为PGI2和PGH1转化为HHD的反应似乎有所不同：在后者反应中底物可用性是限速因素，而酶与PGH2迅速饱和，在较高底物水平下观察到前列环素形成的稳定速率。
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