关于猪主动脉微粒体将前列腺素内过氧化物酶促转化为前列环素的进一步研究。

Further studies on the enzymatic conversion of prostaglandin endoperoxide into prostacyclin by porcine aorta microsomes.

作者信息

Salmon J A, Smith D R, Flower R J, Moncada S, Vane J R

出版信息

Biochim Biophys Acta. 1978 Mar 14;523(1):250-62. doi: 10.1016/0005-2744(78)90028-1.

Abstract

A simple, rapid radiochemical assay for prostacyclin synthesis has been used to characterize the enzyme in arterial walls which converts prostaglandin endoperoxides to prostacyclin. The enzyme displays a broad pH optimum, and catalyses a rapid conversion of saturating concentrations of the endoperoxide at 37 degrees C. Hydroperoxides of several unsaturated fatty acids are potent inhibitors of the enzyme, and act in a time dependent manner. The isomerase which converts prostaglandin endoperoxides to prostaglandin E2 or D2 was not detected in the arterial wall.

摘要

一种用于前列环素合成的简单、快速放射化学测定法已被用于表征动脉壁中能将前列腺素内过氧化物转化为前列环素的酶。该酶具有较宽的最适pH值，在37℃下能催化饱和浓度的内过氧化物快速转化。几种不饱和脂肪酸的氢过氧化物是该酶的有效抑制剂，且作用具有时间依赖性。在动脉壁中未检测到将前列腺素内过氧化物转化为前列腺素E2或D2的异构酶。