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基于冷冻断裂数据的心脏和网膜毛细血管内皮细胞超微结构组织特征

[Characteristics of the ultrastructural organization of endothelial cells in cardiac and omental blood capillaries based on cryofractographic data].

作者信息

Gusev S A, Mironov V A, Povaliĭ T N

出版信息

Tsitologiia. 1980 Feb;22(2):134-8.

PMID:6992367
Abstract

Using cryofractography, endotheliocytes of heart and omentum blood capillaries were studied, different patterns of distribution of membranous particles being observed of the "P" and "E" faces of the fracture plans of endothelial cell cytomembranes. Three types of distribution of micropinocytotic vesicles can be distinguished: the linear, hexagonal, and the clusteral type. The number of micropinocytotic vesicles was sharply reduced near the region of intercellular contacts. On the fracture plan, attached pinocytotic vesicles look like "craters" on the "E" face, and like circumvallatue papillae on the "P" face. Cell membranes, making intercellular contacts, have a network structure consisting of ridges on the "P" face and of grooves on the "E" face. Such a molecular membrane organization is discussed from the standpoint of modern concepts of the receptor--cytosceletal complex. It is supposed that this complex may control processes of transendothelial mass--transfer.

摘要

采用冷冻断裂术研究了心脏和网膜毛细血管的内皮细胞,在内皮细胞质膜断裂面的“P”面和“E”面上观察到膜颗粒的不同分布模式。微吞饮小泡的分布可分为三种类型:线性、六边形和簇状。在细胞间接触区域附近,微吞饮小泡的数量急剧减少。在断裂面上,附着的吞饮小泡在“E”面上看起来像“火山口”,在“P”面上像围堤状乳头。形成细胞间接触的细胞膜具有由“P”面上的嵴和“E”面上的沟组成的网络结构。从受体 - 细胞骨架复合体的现代概念角度讨论了这种分子膜组织。据推测,这种复合体可能控制跨内皮物质转运过程。

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